The use of human antiviral testing is required to foil the spread of pandemic diseases and formulate strategies that'll help eliminate and treat various viral infections. Human antiviral testing is a laboratory process where the existing and potential drugs are tested for their efficiency in fighting viral infections caused by pathogens such as Herpes, Influenza, Hepatitis, and HIV, among many others.
Microbiology Tests
Microbiology tests are used to detect the presence or absence of microorganisms (bacteria, fungi, parasites, and viruses) in various specimens such as blood, urine, body tissues, stool, and respiratory secretions.
A procedure called culture is the gold standard for the diagnosis of infections, especially for bacteria, because it provides a direct measure of the number of organisms in the sample and their susceptibility to different antibiotics. However, culture methods may take days or even weeks to obtain results depending on the type of organism that is being cultured.
To provide faster results and allow clinicians to initiate treatment sooner than later, microbiology tests can be used to directly detect and identify disease-causing microorganisms in minutes or hours using molecular assays such as PCR (polymerase chain reaction), nucleic acid sequencing (NGS), immunoassays such as ELISA (enzyme-linked immunosorbent assay), immunofluorescence; or by using antigen detection methods such as agglutination test, latex agglutination test, and fluorescence microscopy.
Molecular Biology Lab Services
RT-PCR
Real-time PCR (real-time polymerase chain reaction), also known as quantitative PCR (qPCR), is a laboratory technique based on the polymerase chain reaction, which enables rapid amplification and quantification of short segments of DNA. Real-time PCR allows monitoring of the amplification process in real-time by detection of the increase in fluorescence as the PCR products accumulate during each thermal cycle. The results are an accurate genetic fingerprint that can be used to:
• Detect infectious organisms
• Verify product identity
• Assess levels of gene expression
• Identify bacteria
RACE-PCR
RACE-PCR is a technique that allows you to identify the terminal sequences of an mRNA. This technique can be used to identify the 5' and 3' ends of a transcript or for the identification of alternative splice sites in transcripts.
Plasmid Prep
Plasmid DNA prep is a technique used to isolate and purify bacterial plasmids for use in recombinant DNA applications. Plasmid preps are one of the most commonly performed lab protocols and are essential in any molecular biology setting.
qRT-PCR
The qRT-PCR is a technique used to identify and quantify a specific RNA target of interest in a sample. The qRT-PCR is used to measure gene expression levels or the relative abundance of an RNA target in samples compared to reference controls. Targeted RNA quantification provides rapid, reproducible results with minimal RNA input.
Molecular biology lab services are most comprehensive in terms of methodologies and techniques used. Commercial service providers offer standard molecular biology services suitable to all your requirements. With the rapid advancement in molecular biology techniques, antiviral testing, and tools, the more challenging molecular biology experiments need to be outsourced. This reduces time and cost while assuring accuracy and quality.
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